Microsporogenesis, Pollen Ornamentation, Viability of Stored Taxodium distichum var. distichum Pollen and Its Feasibility for Cross Breeding

Taxodium Rich is well known for its flooding tolerance and has great ecological economic potential. A comprehensive understanding of pollen characteristics storage capacity important breeding genetic resource conservation the genus. In this study, we observed microsporogenesis ornamentation, studied conditions in vitro germination, compared difference viability T. distichum var. measured by germination TTC staining, analyzed change after different times feasibility using stored cross breeding. Results indicated that mother cells begin to enter meiosis stage one month before male strobilus disperse, reach metaphase 10 days form grains three five tetrad development. Pollen rate topped culture medium 0.012% boric acid under 30 °C 48 h, reaching 66.81%. The staining demonstrated TD-4 TD-5 were 97.78% 80.54%, 98.96% 91.67%, 83.67% 21.75% one-, two- three-year at −20 °C, which significantly higher than (p < 0.05) 17.02 27.04%, 2.77% 12.82%, 0 determined cultivation. It feasible use year artificial hybridization, fruit setting seed 10.94 36.79%, 11.47 65.76%, respectively.